Optimization of Process for Enzymatic Resolution of Racemic Amines using Continuous Flow Bioreactor

Authors

  • Elazar Meyer Hebrew University, Israel

DOI:

https://doi.org/10.47577/technium.v20i.10794

Keywords:

Optimization, process, continuous flow bioreactor, enzymatic resolution, racemic mixtures, chemical purity and enantiomeric excess.

Abstract

The optimization of enzymatic process for the resolution of chemically and pharmaceutically important racemic amines is described using a continuous flow bioreactor. Also described are the isolation and purification methods of the reaction products and the various parameters affecting the optimal reaction conditions for the enzymatic resolution to afford the products in high chemical purity and enantiomeric excess (ee) of over 90%.  

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References

Enzyme-catalyzed processes in organic solvents (enzymatic catalysis in nonaqueous solvents/water in enzyme catalysis/lipases/enzymes in organic media/enzymatic transesterification), A.Zaks and A.M.Klibanov, Proc. Natl. Acad. Sci. USA, 82, 3192-3196, 1985.

Enzymatic lactonization of γ-hydroxyesters in organic solvents: synthesis of optically pure γ-methylbutyrolactone and γ-phenylbutyrolactone, A.L.Gutman, K. Zuoei and A. Boltanski. Tetrahedron Lett., 28, 3861-3864, 1987.

Enzyme-catalyzed enantio-convergent lactonization of γ-hydroxy diesters in organic solvents, A.L.Gutman and T. Bravdo, J. Org. Chem., 54, 4263-4265, 1989.

Lipase-catalyzed preparation of optically active γ-butyrolactones in organic solvents, A..L.Gutman, K. Zuobi and T. Bravdo, J. Org. Chem., 55, 3546-3552, 1990.

Enzymatic formation of lactams in organic solvents, A.L.Gutman, E. Meyer, X. Yue and C. Abell, Tetrahedron Lett., 33, 3943-3946, 1992.

Practical enzymatic resolution of racemic alcohols and amines in organic solvents, A.L.Gutman, E.Shkolnik, E.Meyer, F.Polak, D.Brenner and A.Boltansky, Annals of the N.Y Academy of Science, 799 (Enzyme Engineering XIII), 620-632, 1996.

Nono-fluorinated derivatives of N-propargyl-1-aminoindan and their use as inhibitors of monoamine oxidase, J.Sterling, R.Levy, A.Veinberg, W.Goldenberg, J.Finberg and A.Gutman, US patent 5,481,541.

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Published

2024-03-22

How to Cite

Meyer, E. (2024). Optimization of Process for Enzymatic Resolution of Racemic Amines using Continuous Flow Bioreactor. Technium: Romanian Journal of Applied Sciences and Technology, 20, 56–79. https://doi.org/10.47577/technium.v20i.10794