Our Laboratory Experience: Comparison of Capillary Electrophoresis/Immunosubtraction and Agarose Gel/Immunofixation

Authors

  • Massimo Pieri Department of Experimental Medicine, University of Tor Vergata, Rome, Italy and Lifebrain srl; Viale Roma 190/A, Guidonia Montecelio, Rome, Italy and Clinical Biochemistry, Tor Vergata University Hospital, Rome, Italy
  • Flaminia Tomassetti Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy
  • Caterina Iodice Lifebrain srl; Viale Roma 190/A, Guidonia Montecelio, Rome, Italy
  • Rosa Piazzolla Lifebrain srl; Viale Roma 190/A, Guidonia Montecelio, Rome, Italy
  • Elena Riboldi Lifebrain srl; Viale Roma 190/A, Guidonia Montecelio, Rome, Italy
  • Francesca Capogreco Lifebrain srl; Viale Roma 190/A, Guidonia Montecelio, Rome, Italy
  • Adalgisa Innocenti Lifebrain s.r.l.
  • Maria Loredana Frassanito Lifebrain srl; Viale Roma 190/A, Guidonia Montecelio, Rome, Italy
  • Sergio Bernardini Department of Experimental Medicine, University of Tor Vergata, Rome, Italy and Clinical Biochemistry, Tor Vergata University Hospital, Rome, Italy
  • Graziella Calugi Lifebrain srl; Viale Roma 190/A, Guidonia Montecelio, Rome, Italy

DOI:

https://doi.org/10.47577/technium.v2i7.2067

Keywords:

Serum protein monoclonal component, Immunofixation Electrophoresis, Immunosubtraction Electrophoresis, Laboratory analysis

Abstract

Abstract. Monoclonal gammopathies represent a wide spectrum of related diseases. The common denominator is the presence of a monoclonal protein in the serum or urine, which can be in the form of intact immunoglobulin, immunoglobulin fragments, and/or free light chains (? and ?). We can detect these abnormalities by immunofixation electrophoresis (IFE) in which specific antibodies are overlaid after electrophoresis and the corresponding immunoglobulin. In our study, we compared gel-based and capillary-based serum protein electrophoresis methods to identify and characterize monoclonal immunoglobulins in serum samples. We analyzed 500 serum samples by Sebia Hydragel agarose gel electrophoresis (AGE)/immunofixation electrophoresis (IFE) and CAPILLARYS 2 capillary zone electrophoresis (CZE)/immunosubtraction (IS). AGE/IFE and CZE/IS had an overall agreement of 98% on serum protein electrophoresis. In our hands, AGE/IFE and CZE/IS had the same specificity for detection of monoclonal proteins; however, CZE/IS seems to be more sensitive than AGE/IFE for the detection of some critical cases. However, CZE/IS is an analytically suitable alternative to AGE/IFE, although laboratories should need to combine, for singular samples, both electrophoretic methods in their clinical routine. A combined use of AGE/IFE and CZE/IS is necessary to lead to an accurate diagnosis and clinically error-free results.

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Published

2020-11-30

How to Cite

Pieri, M., Tomassetti, F., Iodice, C., Piazzolla, R., Riboldi, E., Capogreco, F., Innocenti, A., Frassanito, M. L., Bernardini, S., & Calugi, G. (2020). Our Laboratory Experience: Comparison of Capillary Electrophoresis/Immunosubtraction and Agarose Gel/Immunofixation. Technium: Romanian Journal of Applied Sciences and Technology, 2(7), 267–277. https://doi.org/10.47577/technium.v2i7.2067